Kinetic Rationale for Selectivity towards N- and C-terminal Oxygen Dependent Degradation Domain Substrates Mediated by a Loop Region of Hif Prolyl Hydroxylases

KINETIC RATIONALE FOR SELECTIVITY TOWARDS NAND C-TERMINAL OXYGEN DEPENDENT DEGRADATION DOMAIN SUBSTRATES MEDIATED BY A LOOP REGION OF HIF PROLYL HYDROXYLASES Emily Flashman, Eleanor A. L. Bagg, Rasheduzzaman Chowdhury, Jasmin Mecinović, Christoph Loenarz, Michael A. McDonough, Kirsty S. Hewitson and Christopher J. Schofield Chemistry Research Laboratory, Department of Chemistry and the Oxford Centre for Integrative Systems Biology, University of Oxford, Mansfield Road, Oxford, OX1 3TA, U.K. Running title: HIF prolyl hydroxylase substrate selectivity Address correspondence to: Christopher J. Schofield, Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road, Oxford, OX1 3TA; tel.: +44 1865 275625; fax +44 1865 275674; email [email protected]